|
KMID : 0370220010450060708
|
|
Yakhak Hoeji
2001 Volume.45 No. 6 p.708 ~ p.714
|
|
|
Antibody productivity of HBsAg containing both preS2 and S regions expressed in Chinese hamster ovary cells
|
|
정성균/Chung SK
박정민/이상봉/박동우/김동연/김기호/김흥진/Park JM/Lee SB/Park DW/Kim DY/Kim KH/Kim HJ
|
|
|
Abstract
|
|
|
|
Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including preS region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, IY-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S pretein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg mRNA as expected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24kDa, 27kDa, 34kDa and 36kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|